![]() Top: High binding strength on resin with pore diffusion transport conforms to the so-called shrinking core model. The impact of the intraparticle structure of these resins on the mass transport and sorption processes will be investigated thoroughly.įigure 2: CLSM images of monoclonal antibody adsorption and comparison of different mass transport mechanisms. S HyperD is a composite resin comprising rigid backbone of polystyrene coated silica and a pore space filled with polyacrylamide-based hydrogel. The main difference between the types is the chemical structure and the type of attachment to the resin. In the case of Capto S, a dextran polymer with the functional group is introduced into the resin and bound covalently via a multi-point attachment mechanism. For Capto S ImpAct acrylamide polymer chains containing the functional group (ligand) are attached at the chain ends on the resin forming a tentacle-like structure inside the pore, which results in a so-called single-point attachment. Capto S and Capto S ImpAct both have covalently attached polymers on the inside of the pores, increasing the surface area, and therefore ligand density and binding capacity. SP Sepharose FF has “smooth” pores ligands are covalently attached without using grafting techniques. In this doctoral thesis the cation exchange resins SP Sepharose FF, Capto S, Capto S ImpAct and S HyperD will be compared. The impact of the type of mass transport on the adsorption and desorption rate or competitive binding effects of the two-component mixtures on the respective media will be investigated using established experimental techniques The intraparticle mass transport will be evaluated using resins with four distinct types of solid phase characteristics inside the pore space. The core of the PhD thesis will be the investigation of adsorption and desorption processes of two-component systems comprising model proteins of different properties and sizes. Recent studies on two- or multi-component mixtures and their adsorption properties based on one or the other mechanism have mostly been restricted to protein variants or only one type of resin. The dominant driving force depends on the composition of the adsorbent, the properties of the respective proteins as well as the operating conditions, e.g. There is a variety of different resins with diverse stationary phase characteristics available which directly influence the mass transport of the protein species. Intraparticle mass transport is based on pore diffusion, solid-phase diffusion, or a combination of both. The protein adsorption rate is usually controlled by diffusion inside the particles. It is very important to know what is the Tg (Glass Transition Temperature) or HDT (Heat Distortion Temperature) of the resin in order to know what will be the heat resistance of the part.Designing a protein chromatography process requires an understanding of the rate at with which proteins bind to the chromatography media. The resin also determines the thermal limit of the composite part. Resin also helps to transfer mechanical loads from the fibers of reinforcement to the entire composite part. The matrix bonds the fibres of reinforcement together and helps it conform to a desired shape. So what role does resin play? It serves two basic purposes: Once cured, these finished parts are primarily fiber dominant, meaning their physical properties most resemble those of the reinforcement used. This reaction can also be boosted by UV or IR radiations or just by a source of heat.Ĭomposite laminates are a combination of fibre reinforcement and resin. Thermosetting resins are polymers that react thanks to the addition of a catalyst or a hardener causing an exotherm reaction. We offer resins for hand lay-up, spray-up, infusion, RTM, RTM light, pultrusion, etc. ![]() Castro Composites focuses on most of the important thermosetting resins for composite applications. ![]()
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